感染新型鸭呼肠孤病毒雏番鸭和健康雏番鸭的肝蛋白质组学的差异

doi:10.11843/j.issn.0366-6964.2015.05.017

Abstract

Abstract:

The purpose of this paper was to study the pathogenesis of novel duck reovirus（NDRV） infected muscovy duckling.Forty-four healthy muscovy ducklings were divided into treatment group challenged with NDRV and control one injected with Hank′s.Livers were collected from both groups at the 5^th day after challenge.Proteomics of the livers were determined by differential proteomic technology.Differential protein spots between both groups were performed and analyzed by two-dimensional gel electrophoresis and MALDI-TOF-TOF mass spectrometer，and then they were identified by protein search software，Mascot 2.3.02.Twenty-six differential protein spots were separated and identified.Nine down-regulated protein spots and twelve up-regulated protein spots were observed in the liver samples of the treatment after comparison between the two groups.Three protein spots were appeared only in the livers of control and two protein spots are only emerged in the livers of treatment.The results indicate that differential proteomics in livers，which were impacted and damaged by NDRV may provide a new idea of pathogenesis of NDRV infected muscovy duckling.

CLC Number:

S852.659.4

ZHU Guo-zhen，HUANG Mei-qing，CHEN Shi-long，ZHENG Min，CHENG Xiao-xia，CHEN Shao-ying. Differential Proteomics in Livers of Muscovy Ducklings in Health and Challenged with Novel Duck Reovirus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 808-814.

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Differential Proteomics in Livers of Muscovy Ducklings in Health and Challenged with Novel Duck Reovirus

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